Ion exchange chromatography (IEC) is a chromatographic method that uses an ion exchanger as the stationary phase, and separates the component ions in the mobile phase according to the difference in binding force during reversible exchange with the counter ions on the exchanger. Currently, ion exchange chromatography is still a common chromatographic method in the field of biochemistry, and is widely used for the separation and purification of various natural products such as sugars and proteins.
Ion exchange chromatography requires the selection of a suitable chromatographic column depending on the amount of sample to be separated. Chromatographic columns for ion exchange are generally thick and short, and should not be too long.
The ionic strength and pH of the equilibrium buffer and elution buffer in ion exchange chromatography have a great influence on the separation effect.
The eluent buffer should be selected to ensure that all components to be separated are stable over the entire range of the eluent gradient. The gradient range should be as small as possible to improve the resolution.
Most of the ion exchange agents are based on cellulose, spherical cellulose, glucose, etc., which are suitable for the separation of large molecules.
BOC Sciences provides professional ion exchange chromatography for the separation and purification of natural products to help our customers to better develop their products. Our services also include the development of ion exchange chromatography solutions and the optimization of ion exchange chromatography techniques that can be used to efficiently accomplish the separation and extraction of products.
Before performing the separation work, the sample needs to be accommodated using a buffer at a specific pH value.
The sample and column are prepared, the sample is poured into the column, the fluid is expelled down the gel matrix, and the ions are bound to the functional groups of the column and collected as they exit at the bottom.
Elute the components, usually allowing the charge of the molecules in the sample to change or using another substance to replace the bound molecules.
Ion exchange chromatography is also widely used for the separation and purification of small molecules such as inorganic ions, organic acids, nucleotides, amino acids, and antibiotics.
Ion-exchange chromatography is an important tool for the separation and purification of biological macromolecules such as proteins, based on the different charged properties of the substances.
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