Ion Exchange Chromatography Services
Ion exchange chromatography (IEC) is a chromatographic method that uses an ion exchanger as the stationary phase, and separates the component ions in the mobile phase according to the difference in binding force during reversible exchange with the counter ions on the exchanger. Currently, ion exchange chromatography is still a common chromatographic method in the field of biochemistry, and is widely used for the separation and purification of various natural products such as sugars and proteins.
Factors Affecting Ion Exchange Chromatography
Ion exchange chromatography requires the selection of a suitable chromatographic column depending on the amount of sample to be separated. Chromatographic columns for ion exchange are generally thick and short, and should not be too long.
The ionic strength and pH of the equilibrium buffer and elution buffer in ion exchange chromatography have a great influence on the separation effect.
The eluent buffer should be selected to ensure that all components to be separated are stable over the entire range of the eluent gradient. The gradient range should be as small as possible to improve the resolution.
- Matrix of the ion exchanger
Most of the ion exchange agents are based on cellulose, spherical cellulose, glucose, etc., which are suitable for the separation of large molecules.
Our Services
BOC Sciences provides professional ion exchange chromatography for the separation and purification of natural products to help our customers to better develop their products. Our services also include the development of ion exchange chromatography solutions and the optimization of ion exchange chromatography techniques that can be used to efficiently accomplish the separation and extraction of products.
Service steps
Before performing the separation work, the sample needs to be accommodated using a buffer at a specific pH value.
- Ion exchange chromatography
The sample and column are prepared, the sample is poured into the column, the fluid is expelled down the gel matrix, and the ions are bound to the functional groups of the column and collected as they exit at the bottom.
Elute the components, usually allowing the charge of the molecules in the sample to change or using another substance to replace the bound molecules.
Applications of Ion Exchange Chromatography
Separation and purification of small molecules
Ion exchange chromatography is also widely used for the separation and purification of small molecules such as inorganic ions, organic acids, nucleotides, amino acids, and antibiotics.
Separation and purification of biological macromolecules
Ion-exchange chromatography is an important tool for the separation and purification of biological macromolecules such as proteins, based on the different charged properties of the substances.
Our Advantages
- It can be used for almost any type of charged molecule, including large proteins, small nucleotides and amino acids.
- Our professional technicians have extensive experience in the extraction and purification of natural products and provide the most professional service to our customers in the shortest time.
- BOC Sciences provides the most comprehensive services at the best price to help customers save experimental costs reasonably and effectively.
Service Process
About Us
BOC Sciences is an innovator and manufacturer of natural products, and we meet the market demand for natural product monomers. Our expert team has extensive experience in all synthesis stages and has successfully contributed numerous compounds for the fields of medicine, agriculture and environment. The production, testing, packaging and release of all compounds follow strict specifications. If you have a requirement about our services, please contact us by phone or email, our colleagues will reply to you within three working days.
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