High Performance Liquid Chromatography Services

High Performance Liquid Chromatography Services

High performance liquid chromatography (HPLC) has the characteristics of rapid, high efficiency and high resolution, and is widely used in chemical, pharmaceutical, biochemical and environmental protection fields. Due to the high separation capacity and high sensitivity of HPLC, it can be performed at room temperature and has an extremely wide range of applications. Both polar and non-polar, small and large molecules, thermally stable and unstable compounds can be determined by this method, which is especially beneficial for proteins, alkaloids, steroids and lipids in natural products.

Parameters Affecting HPLC

  • HPLC stationary phase

Requirements of HPLC stationary phase: fine particles, regular, narrow diameter range, shallow pores, able to withstand high pressure.

  • Optimization of operating conditions

The optimal combination of separation of components, analysis time and column operating pressure is required.

Our Services

Our Services of High Performance Liquid Chromatography Services

BOC Sciences offers high performance liquid chromatography services for the composition and structure analysis of high boiling point, thermally unstable organic substances, excellent separation of natural products, and fast and accurate quantitative analysis.

Service content

  • Separation of alkaloids
  • Flavonoid separation
  • Terpenoid separation
  • Steroid isolation
  • Other types of natural products

In natural product isolation and purification, BOC Sciences typically combines traditional isolation methods with various classical chromatographic methods for target substances, followed by high performance liquid chromatography for the preparation of monomeric compounds. Our research professionals have also developed a technology integration and sample library for the separation and purification of natural products by HPLC.

Advantages of High Performance Liquid Chromatography

  • High pressure: Liquid chromatography uses liquid as the mobile phase. The liquid flows through the column with high resistance, and high pressure must be applied to the carrier liquid in order to pass through the column quickly.
  • High speed: The flow rate of high performance liquid chromatography is much faster than that of classical liquid chromatography, generally up to 1-10 mL/min, and individually up to 100 mL/min or more, which is similar to that of gas chromatography.
  • High efficiency: The separation efficiency of gas chromatography is very high, and the column efficiency of high performance liquid chromatography is even higher, generally up to about 60,000 theoretical towers/m.
  • High sensitivity: The minimum detection of UV detector can reach the order of nano grams (10-9 g); the sensitivity of fluorescence detector can reach 10-11 g.

Service Process

Service Process of High Performance Liquid Chromatography Services

About Us

BOC Sciences is an innovator and manufacturer of natural products, and we meet the market demand for natural product monomers. Our expert team has extensive experience in all synthesis stages and has successfully contributed numerous compounds for the fields of medicine, agriculture and environment. The production, testing, packaging and release of all compounds follow strict specifications. If you have a requirement about our services, please contact us by phone or email, our colleagues will reply to you within three working days.

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