Gel filtration chromatography, also known as spatial exclusion chromatography, molecular sieve, etc. When the sample solution through the gel column, the relative molecular mass of the larger substances due to the diameter is greater than the gel mesh and only along the gel particles between the gaps, with the solvent flow, so the flow is shorter, moving forward fast and first out of the chromatography column; Conversely, the relative molecular mass of the smaller substances due to the diameter is smaller than the gel mesh, can freely enter and exit the mesh of the gel particles, in the process of moving down, they from the gel In the process of downward movement, they diffuse from the gel to the pores of the gel particles and then enter another gel particle, and so on continuously enter and escape, so that the flow rate grows and the movement rate is slow and finally out of the chromatography column. As for the medium-sized molecules, they can also be distributed inside and outside the gel particles, partially entering the particles and thus being eluted between the large and small molecules. Usually, most gel matrices are prepared from chemically cross-linked polymer molecules, and the degree of cross-linking determines the pore size of the gel particles.
Requirements for gels: chemically inert; containing few ionic groups; uniform mesh and particle size; suitable gel particle size and mesh size, wide range of options available; good mechanical strength.
Choice of solvent: It can not only dissolve the sample but also wet the gel, with high purity, low toxicity, good solubility, it can dissolve a variety of macromolecules, and the viscosity is as low as possible.
BOC Sciences can separate molecules and complexes in natural products into fractions with a specific size range by gel filtration chromatography to remove all molecules larger than a specific size from the sample. Gel filtration chromatography can be used to separate small molecules, proteins, polysaccharides and other compounds in aqueous solutions.
BOC Sciences offers gel filtration chromatography with the following advantages.
BOC Sciences is an innovator and manufacturer of natural products, and we meet the market demand for natural product monomers. Our expert team has extensive experience in all synthesis stages and has successfully contributed numerous compounds for the fields of medicine, agriculture and environment. The production, testing, packaging and release of all compounds follow strict specifications. If you have a requirement about our services, please contact us by phone or email, our colleagues will reply to you within three working days.