Amino Acid & Protein Extraction Services
According to the nature of amino acids, which are easily soluble in water and insoluble in organic solvents, they are often extracted by water leaching and dilute ethanol leaching methods. The aqueous solutions of amino acids produced by protein hydrolysis and leaching methods often contain several and dozens of amino acids, so the resulting amino acids must be separated and purified. The methods often used are chromatography (distribution column chromatography, filter paper chromatography, thin layer chromatography, ion exchange chromatography, gas chromatography, high pressure liquid chromatography), salt formation separation, crystal analysis, etc. Proteins are large molecules composed of long-chain amino acids. They include enzymes, cell signaling proteins, ligand binding proteins, and cytoskeletal proteins. In order to obtain high protein yields, samples (e.g. plant and animal tissues, cultured cells) must be homogenized uniformly and efficiently and ensure that researchers always obtain the highest protein yields and purity.
Our Services
BOC Sciences offers professional amino acid and protein extraction services to extract natural products from plants, animals or cultured cells. After we provide an effective solution, we believe we can help you complete your product development as quickly as possible for a wide range of applications in the pharmaceutical, food and daily chemical industries.
Amino acid extraction services
After crushing herbs with a pulverizer, they are loaded into each leaching tank of the counter-current leaching tank set, using water as the leaching solvent, and heated for counter-current leaching under stirring conditions.
- Dilute ethanol leaching method
The raw drug powder is loaded into each leaching tank of the counter-current leaching tank group and leached by percolation with 70% ethanol, with the solution leached first being more concentrated and the solution leached later being less concentrated.
Protein extraction services
The protein extraction process starts with very coarse samples which are purified by filtration, centrifugation, dissolution and precipitation and refined using techniques such as affinity columns and immunoprecipitation.
Workflow
- Cells are lysed by sonication or the tissue is homogenized by freezing and grinding or homogenization. This process disrupts the cell walls and releases proteins into the sample. Tissue samples can then be filtered to remove large debris.
- Centrifuge the sample to remove cellular debris. This is usually done by differential centrifugation or density gradient centrifugation in an ultracentrifuge.
- For proteins that will be used for denaturing gel electrophoresis or protein blotting - precipitate the protein using acetone.
- Resuspend the sample in the appropriate protein extraction buffer. This buffer will contain the appropriate amount of salt and will contain any necessary detergent, reducing agent, denaturant or protease inhibitor. Detergents help to solubilize insoluble proteins, reducing agents reduce oxidative damage, and denaturants are used when the goal is to denature the protein for molecular weight analysis. Once the membrane is damaged and everything is suddenly mixed together, protease inhibitors prevent protease degradation.
- Further refine the target protein solution.
Elements of Attention in Protein Extraction
Protein extraction is the process of isolating and purifying proteins from whole tissue, cell culture or biological fluid samples. The protein extraction protocol used is customized to match the starting material and the end goal of the analysis. It is important to consider the goals of the experiment when developing a protein extraction protocol because certain buffer choices (e.g., high salt, high detergent formulations) may disrupt the experiment when higher-order protein structure and function need to be preserved.
If you have a requirement about our services, please contact us by phone or email, our colleagues will reply to you within three working days.
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