According to the nature of amino acids, which are easily soluble in water and insoluble in organic solvents, they are often extracted by water leaching and dilute ethanol leaching methods. The aqueous solutions of amino acids produced by protein hydrolysis and leaching methods often contain several and dozens of amino acids, so the resulting amino acids must be separated and purified. The methods often used are chromatography (distribution column chromatography, filter paper chromatography, thin layer chromatography, ion exchange chromatography, gas chromatography, high pressure liquid chromatography), salt formation separation, crystal analysis, etc. Proteins are large molecules composed of long-chain amino acids. They include enzymes, cell signaling proteins, ligand binding proteins, and cytoskeletal proteins. In order to obtain high protein yields, samples (e.g. plant and animal tissues, cultured cells) must be homogenized uniformly and efficiently and ensure that researchers always obtain the highest protein yields and purity.
BOC Sciences offers professional amino acid and protein extraction services to extract natural products from plants, animals or cultured cells. After we provide an effective solution, we believe we can help you complete your product development as quickly as possible for a wide range of applications in the pharmaceutical, food and daily chemical industries.
After crushing herbs with a pulverizer, they are loaded into each leaching tank of the counter-current leaching tank set, using water as the leaching solvent, and heated for counter-current leaching under stirring conditions.
The raw drug powder is loaded into each leaching tank of the counter-current leaching tank group and leached by percolation with 70% ethanol, with the solution leached first being more concentrated and the solution leached later being less concentrated.
The protein extraction process starts with very coarse samples which are purified by filtration, centrifugation, dissolution and precipitation and refined using techniques such as affinity columns and immunoprecipitation.
Protein extraction is the process of isolating and purifying proteins from whole tissue, cell culture or biological fluid samples. The protein extraction protocol used is customized to match the starting material and the end goal of the analysis. It is important to consider the goals of the experiment when developing a protein extraction protocol because certain buffer choices (e.g., high salt, high detergent formulations) may disrupt the experiment when higher-order protein structure and function need to be preserved.
If you have a requirement about our services, please contact us by phone or email, our colleagues will reply to you within three working days.